Page 190 - MaterialsTrial-JapaneseArmy-1950

Question: How much time was required for the breeding of bacteria of each type: typhoid,
cholera, anthrax, plague.

Answer: Plague and anthrax germs required 48 hours, cholera, typhoid and other germs
required 24 hours.

Question: What was the yield of anthrax germs per cultivator?

Answer: From 50 to 60 grams.

Question: And the yield of typhoid germs per cultivator?

Answer: From 40 to 45 grams.

Question: Plague?

Answer: Thirty grams.

Question: Cholera?

Answer: About 50 grams.

Question: How were the germs produced by the 4th Division preserved?

Answer: If for a short period, they were preserved in refrigerators.

Question: When you despatched germs for the bacteriological attacks on the Chinese
population, how were the germs packed?

Answer: The germs were put into special bottles, each of which could hold 50 grams. The
bottles were put into metal containers, which were packed into large special cases lined with
ice, several containers to a case.

Question: Will you describe the methods and the special equipment employed by
Detachment 731 for the largescale breeding of fleas?

Answer: The 2nd Division had four special premises for the mass breeding of fleas, in
which a fixed temperature of 4-30°C. was maintained. Metal jars, 30 cm. high and 50 cm.
wide, were used for the breeding of the fleas. Rice husks were poured into the- jars to keep
the fleas in. After these preparations, a few fleas were put into each jar, and also a white rat
for them to feed on. The rat was fastened in such a way as not to hurt the fleas. A constant
temperature of -f-30°C. was maintained in the jars.

Question: What quantity of fleas could be obtained from each cultivator in one production
cycle?

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